150 μm Magnetic Beads Protein Purification 10% Volume Ratio 5 mL

Price	Negotiation
MOQ	5 mL / bottle
Delivery Time	5-8 days
Brand	BeaverBeads
Place of Origin	China
Certification	COA
Model Number	70805-5
Packaging Details	5mL/100mL/500mL
Payment Terms	L/C, T/T
Supply Ability	850 L/month

Product Details

Product Specification

Usage	Medical Research	Place of Origin	China
Packaging Details	5mL/100mL/500mL	Product Name	Magrose Protein G Magbeads
Shelf life	2 years	Specification	5 mL, 10% (v/v), 30-150 μm
Concentration	10% (v/v)	Model Number	70805-5
Supply Ability	850 L/month	Certification	COA
Brand Name	BeaverBeads	Payment Terms	L/C, T/T
Storage	2~8℃ sealed storage	Price	Negotiation
Delivery Time	5-8 days	Minimum Order Quantity	5 mL / bottle
Package	5mL/100mL/500mL	Application	Protein Purification

Product Description

Magrose Protein G Magbeads for Protein Purification 30-150 μm, 10% (v/v)

1, Description:

This product is micron magnetic beads, skilled operation can complete the antibody adsorption process within 15 min, and complete the process of antibody purification within 30 min. The one-step solution can reduce the antibody absorption process to 10 minutes and purification process to 30 minutes, yielding antibody with >90% purity. It can be used for antibody fixation and other related studies. By referring the attached Table 1 users can select the categories of magnetic beads according to the source and subtype of the target antibody, and compare the affinity of Magrose Protein A and Magrose Protein G beads with different antibodies.

2, Product Information:

Product Name	Magrose Protein A
Bead Size	30~150 μm
Beads Concentration	10% (v/v)
Ligand	Protein A
Medium	Magrose
Antibody Bonding Quantity	25~30 mg Human IgG/mL Gel
Storage Temperature	2~8℃
Binding/Washing Buffer	PBST (pH 7.2~7.4): 137 mM NaCl,2.7 mM KCl, 10 mM Na₂HPO₄, 2.0 mM KH₂PO4, 0.1% Tween-20
Elution buffer	100 mM Gly, 0.1% Tween-20, pH 2.5
Neutrilization buffer	1.0 M Tris-HCl, pH 9.0
Storage buffer	PBST (includes 0.05% NaN3)
Shelf life	12 months

3, Feature:

1. Highly effective antibody binding capacity and ultra-low non-specific adsorption performance

2. Time saving, simple and mild

3. High product stability

4. Antibody elution system is closer to neutral

5. Very low protein A ligand off rate

6. Reusable

4, FAQ:

Q1: How to avoid possible aggregation of magnetic beads during storage or use?

A1: Beads should be stored at 2~8℃, and avoid for irreversible aggregation due to contamination or drying. Magnetic beads aggregation at low pH elution buffer is a normal phenomenon, and it does not affect the normal use of magnetic beads. In Binding/Washing buffer and Elution buffer adding with the final concentration of 0.1% (v/v) nonionic detergent (such as NP-40, Tween-20 or Triton X-100) can effectively prevent the beads

aggregation. The magnetic beads in low pH elution can be washed to neutral with Binding/Washing buffer and Elution buffer, and process with 2 min ultrasonic water bath. It can restore the magnetic beads to homogeneity, and the above treatments had no effect on the antibody binding efficiency with magnetic beads.

Q2: Magnetic beads appear agglomeration in the process of using?

A2: The agglomeration phenomenon is generally difficult to shake and break, which easily lead to uneven distribution. The reason is mainly because the magnetic beads are placed in the magnetic field for too long, the magnetic beads firmly binding together. The magnetic beads can be dispersed by 2 min ultrasonic water bath, but it should be noted that the antibody captured in the sample solution can lost from ultrasonic treatment, so it’s not recommended to use this method until elution.

150 μm Magnetic Beads Protein Purification 10% Volume Ratio 5 mL

Product Details

Product Specification

Product Description

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